MonoRab™ GS Linker Antibody [iFluor 647], mAb, Rabbit：This product recognizes (GnS)m linker(n≥2, m≥2) in proteins。

产品型号：A02311

更新时间：2025-05-19

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产品介绍

This product recognizes (GnS)m linker(n≥2, m≥2) in proteins, antibodies or cells, such as (G2S)2 linker, (G2S)4 linker, (G3S)3 linker and (G4S)3 linker, etc.

产品概述

Specificity	This product recognizes (GnS)m linker(n≥2, m≥2) in proteins, antibodies or cells, such as (G2S)2 linker, (G2S)4 linker, (G3S)3 linker, (G4S)2 linker and (G4S)3 linker, etc.
Host Species	Rabbit
Immunogen	A synthetic peptide (GGGGSGGGGSGGGGS) coupled to BSA
Conjugate	iFluor 647

应用指导

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
Flow Cytometry	1:50

产品属性

Form	Liquid
Storage Buffer	Supplied in PBS (pH 7.2), containing 10 mg/ml BSA, 0.03% ProClin300 and 50% glycerol.
Storage Instructions	Store at -20°C and it should be protected from prolonged exposure to light. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Protein A affinity column
Isotype	Rabbit IgG, κ
Clonality	Monoclonal
Clone ID	20H7
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

图例

技术背景

Target Background	A linker is an amino acid chain that connects two fused proteins, allowing for the appropriate separation of protein functional domains or the maintenance of necessary inter-domain interactions. The most commonly used flexible linker is the poly-Glycine-Serine (GS) linker. A very important application of the flexible GS linker is connecting the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs). Due to its flexible structure and appropriate linker sequence length, the (GGGGS)3 linker has been shown to allow for the correct orientation of the VH and VL domains, and it does not interfere with the folding of the protein domains in scFv construction[1,2].
Synonyms	GS Linker;Glycine-serine linker; (G4S)3 Linker; poly-Glycine-Serine; (GGGGS)3;
Reference	[1] Huston,J,S,et al.Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.[J].Proceedings of the National Academy of Sciences, 1988, 85(16):5879-5883. [2] Chen X , Zaro J ,Shen, Wei‐Chiang.Fusion Protein Linkers: Effects on Production, Bioactivity, and Pharmacokinetics[M].John Wiley & Sons, Inc. 2013.

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